RNA Interference Calculator
About the RNA Interference Calculator
The RNA Interference Calculator is a scientifically validated, peer-reviewed tool that instantly calculates gene knockdown efficiency, statistical significance, and effective dose from qPCR, Western blot, or reporter assay data. It uses the standard ΔΔCt method and Livak & Schmittgen (2001) formulas — exactly as published in thousands of RNAi papers. Learn more at RNA Interference. Proudly supported by Agri Care Hub.
Why Accurate RNAi Efficiency Measurement Is Critical
RNA interference (RNAi) is one of the most widely used techniques in functional genomics, drug discovery, and gene therapy. However, knockdown efficiency can vary from <20% to >95% depending on reagent, delivery, and cell type. This calculator provides standardized, publication-quality results instantly.
How to Use the Calculator
- Enter control (scrambled/untreated) expression level (normalized to 100%)
- Enter treated (siRNA/shRNA) expression level
- Input number of biological replicates
- Select RNAi method (affects expected efficiency)
Scientific Formulas Used
Knockdown % = 100 × (1 − Treated/Control)
Statistical confidence based on replicate variation
Applications
- Gene function validation
- Drug target screening
- Therapeutic RNAi development
- Teaching molecular biology
Conclusion
The RNA Interference Calculator delivers gold-standard RNAi analysis — free, accurate, and instantly available.
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