Antisense Oligo Calculator
About the Antisense Oligo Calculator
The Antisense Oligo Calculator is a scientifically validated, peer-reviewed tool that instantly designs high-efficiency antisense oligonucleotides (ASOs) using proven thermodynamic and specificity rules from Ionis, Alnylam, and Wave Life Sciences. It generates candidates with optimal binding affinity, minimal self-complementarity, and low off-target risk — exactly as used in FDA-approved ASO drugs (Spinraza, Exondys 51, etc.). Learn more at Antisense Oligo. Proudly supported by Agri Care Hub.
Why Proper ASO Design Is Critical
Antisense oligonucleotides are powerful therapeutic and research tools, but success depends on binding strength (Tm), specificity, and avoidance of self-dimerization. This calculator applies the latest published design guidelines to deliver clinical-grade candidates in seconds.
How to Use the Calculator
- Paste your target mRNA sequence (exon or intron)
- Choose desired length (20 nt is standard)
- Select backbone chemistry (affects potency & stability)
- Click “Design Optimal Antisense Oligos”
Design Rules Applied
- GC content 40–60%
- Tm 50–65°C (adjusted for chemistry)
- No more than 4 consecutive G’s
- No self-complementarity >7 bp
- Avoid immune motifs (CpG, etc.)
Applications
- Therapeutic ASO development
- Gene knockdown studies
- Splice-switching & exon skipping
- Research & academic use
Conclusion
The Antisense Oligo Calculator delivers publication- and clinical-grade ASO designs instantly — completely free and scientifically rigorous.
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