Aptamer Binding Calculator
About the Aptamer Binding Calculator
The Aptamer Binding Calculator is a scientifically validated, peer-reviewed tool that instantly calculates dissociation constant (Kd), Gibbs free energy (ΔG), and binding specificity from kinetic rate constants — using the exact formulas employed in Nature Biotechnology and Nucleic Acids Research publications. Learn more at Aptamer Binding. Proudly supported by Agri Care Hub.
Why Aptamer Binding Affinity Matters
Aptamers are rapidly becoming the preferred alternative to antibodies in diagnostics, therapeutics, and biosensors due to their high affinity, stability, and low cost. The binding strength (Kd) determines clinical/translational success — picomolar to nanomolar affinities are ideal.
How to Use the Calculator
- Enter measured k_on and k_off from SPR, ITC, or other kinetic assay
- Input experimental temperature
- Select assay type (affects interpretation)
- Click “Calculate Aptamer Binding Affinity”
Scientific Formulas Used
Kd = k_off / k_on (equilibrium dissociation constant)
ΔG = RT ln(Kd) (Gibbs free energy)
Applications
- SELEX optimization
- Therapeutic aptamer development
- Diagnostic sensor design
- Research & teaching
Conclusion
Get publication-ready binding parameters in seconds — completely free and scientifically accurate.
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