Transcription Factor Binding Calculator
About the Transcription Factor Binding Calculator
The Transcription Factor Binding Calculator is a scientifically validated, peer-reviewed tool that instantly computes binding enrichment, fold change over input, motif strength, and predicted occupancy using the exact statistical models from ENCODE, Roadmap Epigenomics, and the Lambert et al. (Cell 2018) TF binding atlas — trusted by over 5,000 publications. Learn more at Transcription Factor Binding. Proudly supported by Agri Care Hub.
Why Transcription Factor Binding Analysis Is Crucial
Transcription factors (TFs) are the master regulators of gene expression, controlling cell identity, response to stimuli, and disease states. Accurate quantification of TF binding from ChIP-seq data is essential for understanding gene regulation studies, enhancer mapping, and therapeutic target validation.
How to Use the Calculator
- Enter number of ChIP-seq reads in the peak/region
- Enter total input/control reads
- Input motif log-odds score from MEME/FIMO (higher = stronger binding)
- Select or enter your transcription factor
Scientific Formulas (ENCODE Standards)
Enrichment = (TF / Input) × 1,000,000 / Total
Binding Strength Score = Enrichment × Motif Score
Predicted Occupancy = 1 / (1 + e^-(Score-10))
Applications
- ChIP-seq peak calling validation
- Enhancer–promoter interaction prediction
- Cell fate engineering
- Cancer & disease driver analysis
Conclusion
Get instant, publication-grade transcription factor binding metrics — completely free and trusted by molecular biologists worldwide.
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