All Biochemistry Calculators Related Post

Antigen-Antibody Binding Calculator Binding Affinity Calculator (Kd Estimation) This calculator estimates the dissociation constant (Kd) for antigen-antibody binding using a standard ELISA titration method, a peer-reviewed approach based on hyperbolic binding curve fitting. The Kd is the antigen concentration at half-maximal binding signal. Maximum Signal (Amax, e.g., highest absorbance): Background Signal (Amin, no antigen or […]

Complement Activation Calculator CH50 Complement Activity Calculator (Classical Pathway) This calculator estimates total classical complement pathway activity (CH50) using data from the standard hemolytic assay, a peer-reviewed gold standard method for assessing functional complement activation. Enter absorbance values from your serum dilutions to compute the CH50 value. Enter absorbance at 541 nm (or 415 nm)

Cytokine Release Calculator Cytokine Concentration Calculator This calculator determines cytokine concentration using the standard ELISA interpolation from a linear standard curve, a peer-reviewed and widely accepted method for quantifying cytokines (e.g., IL-6, TNF-α) in cell culture supernatants, serum, or plasma. It assumes a linear relationship between absorbance and concentration in the working range. Sample Absorbance

Inflammation Marker Calculator Inflammation Markers Calculator This scientifically validated calculator computes key inflammation indices from standard blood tests: Neutrophil-to-Lymphocyte Ratio (NLR), Platelet-to-Lymphocyte Ratio (PLR), Systemic Immune-Inflammation Index (SII), and C-Reactive Protein to Albumin Ratio (CAR). These peer-reviewed markers help assess systemic inflammation levels. C-Reactive Protein (CRP, mg/L): Albumin (g/L): Neutrophils (×10⁹/L): Lymphocytes (×10⁹/L): Platelets (×10⁹/L):

Oxidative Burst Calculator Oxidative Burst Intensity Calculator This calculator quantifies the oxidative burst (also known as respiratory burst) using data from the standard luminol-peroxidase chemiluminescence assay, a peer-reviewed method widely used to measure reactive oxygen species (ROS) production in plant and animal immune cells. Peak RLU from Elicited Sample: Peak RLU from Control (unstimulated): Tissue

Phagocytosis Rate Calculator Phagocytosis Rate Calculator This calculator computes key phagocytosis metrics using the standard microscopic assay method, a peer-reviewed and widely accepted technique in immunology. It calculates the Percentage of Phagocytic Cells and the Phagocytic Index based on manual counts from stained slides (e.g., opsonized sheep red blood cells or fluorescent beads with macrophages/neutrophils).

Cell Adhesion Calculator Cell Adhesion Percentage Calculator This calculator computes the percentage of cell adhesion using standard protocols from peer-reviewed sources, such as fluorescent or colorimetric (e.g., crystal violet) assays. The formula is: Adhesion % = (Measure of Adherent Cells / Measure of Total Cells) × 100. Measure of Adherent Cells (e.g., OD570 or Fluorescence

Integrin Binding Calculator Integrin Binding Affinity Calculator (Ki from IC50) This calculator computes the inhibition constant (Ki) from IC50 values using the Cheng-Prusoff equation, a standard peer-reviewed method widely applied in integrin-ligand binding studies (e.g., competition assays with RGD peptides). IC50 of Inhibitor (nM): Concentration of Competing Ligand (nM): Kd of Competing Ligand (nM): Calculate

Chemotaxis Calculator Chemotaxis Index Calculator This calculator computes the Chemotaxis Index (CI) using the standard population-based formula widely used in peer-reviewed studies on C. elegans, bacteria, and leukocytes. The formula CI = (A – B) / (A + B) provides a reliable measure of directional migration, ranging from +1 (strong attraction) to -1 (strong repulsion).

Signal Transduction Calculator Dose-Response Curve Analyzer (Hill Equation) This calculator uses the Hill equation, a cornerstone of pharmacology and signal transduction research, to determine key parameters like EC₅₀ (half-maximal effective concentration), Hill coefficient (n_H, indicating cooperativity), and maximal response from dose-response data. It is widely applied in receptor-ligand binding and downstream signaling pathway analysis. Assumed