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Enzyme Turnover Calculator

Enzyme Turnover Calculator

Enzyme Turnover Calculator Calculate Enzyme Turnover Number (k_cat) V_max (Maximum Reaction Rate): [E]_T (Total Enzyme Concentration): Calculate k_cat About the Enzyme Turnover Calculator The Enzyme Turnover Calculator is a scientifically accurate tool designed to compute the turnover number (k_cat), a fundamental parameter in enzyme kinetics. The turnover number represents the maximum number of substrate molecules […]

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Peroxidase Activity Calculator

Peroxidase Activity Calculator

Peroxidase Activity Calculator Calculate Peroxidase Activity Absorbance at 470 nm (ΔA₄₇₀): Reaction Volume (mL): Enzyme Volume (mL): Extinction Coefficient (mM⁻¹ cm⁻¹): Path Length (cm): Calculate Activity About the Peroxidase Activity Calculator The Peroxidase Activity Calculator is a scientifically accurate, user-friendly tool designed to quantify peroxidase enzyme activity in biological samples using the standard guaiacol assay

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Catalase Activity Calculator

Catalase Activity Calculator

Catalase Activity Calculator Calculate Catalase Activity Change in Absorbance (ΔA) at 240 nm: Time Interval (seconds): Reaction Volume (mL): Enzyme Volume (mL): Extinction Coefficient (mM⁻¹ cm⁻¹): Standard value for H₂O₂ at 240 nm is 0.0436 mM⁻¹ cm⁻¹ Cuvette Path Length (cm): Calculate Activity About the Catalase Activity Calculator The Catalase Activity Calculator is a precision-engineered

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Superoxide Dismutase Calculator

Superoxide Dismutase Calculator

Superoxide Dismutase Calculator Calculate SOD Activity Absorbance of Control (A₍control₎): Absorbance of Sample (A₍sample₎): Dilution Factor (if any): Sample Volume (mL): Calculate SOD Activity About the Superoxide Dismutase Calculator The Superoxide Dismutase Calculator is a scientifically accurate tool designed to measure the activity of Superoxide Dismutase (SOD), a critical antioxidant enzyme that protects cells from

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Redox Balance Calculator

Redox Balance Calculator

Redox Balance Calculator Calculate Cellular Redox Balance (NAD+/NADH Ratio) NAD⁺ Concentration (μM): NADH Concentration (μM): Calculate Redox Balance About the Redox Balance Calculator The Redox Balance Calculator is a scientifically accurate tool designed to determine the cellular redox state by calculating the NAD⁺/NADH ratio, a critical biomarker of metabolic health and energy homeostasis. This ratio

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Oxidative Stress Calculator

Oxidative Stress Calculator

Oxidative Stress Calculator Sample Type PlasmaSerumTissue HomogenateCell Lysate Reactive Oxygen Species (ROS) Level (µM H₂O₂ equivalent) Superoxide Dismutase (SOD) Activity (U/mL or U/mg protein) Catalase (CAT) Activity (µmol/min/mL) Glutathione Peroxidase (GPx) Activity (mU/mL) Reduced Glutathione (GSH) Level (µM) Protein Concentration (mg/mL) (for tissue/cell samples) Calculate Oxidative Stress Index Oxidative Stress Assessment Results About the Oxidative

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Glutathione Ratio Calculator

Glutathione Ratio Calculator

Glutathione Ratio Calculator Reduced Glutathione (GSH) Concentration (µM) Oxidized Glutathione (GSSG) Concentration (µM) Calculate Glutathione Ratio Results About the Glutathione Ratio Calculator The Glutathione Ratio Calculator is a precision biochemical tool designed to determine the redox status of cells by calculating the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG). This ratio is a

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GSH/GSSG Calculator

GSH/GSSG Calculator

GSH/GSSG Calculator GSH Concentration (µM) GSSG Concentration (µM) Sample Volume (µL) Cell Count (×10⁶ cells) Calculate Redox State Results About the GSH/GSSG Calculator The GSH/GSSG Calculator is a precision biochemical tool designed for researchers, biochemists, and cell biologists to accurately determine the cellular redox state using reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations. Based

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ROS Production Calculator

ROS Production Calculator

ROS Production Calculator Cell Type HepatocyteNeutrophilMacrophageFibroblastEndothelial CellNeuron Stimulus / Condition Basal (Unstimulated)PMA (100 nM)LPS (1 µg/mL)H₂O₂ (100 µM)Hypoxia (1% O₂)TNF-α (10 ng/mL) Exposure Time (minutes) Cell Count (×10⁶ cells) Detection Method DCFH-DA (Fluorescence)Luminol (Chemiluminescence)MitoSOX Red (Mitochondrial)Amplex Red (H₂O₂ Specific) Calculate ROS Production ROS Production Results About the ROS Production Calculator The ROS Production Calculator is

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Antioxidant Capacity Calculator

Antioxidant Capacity Calculator

Antioxidant Capacity Calculator Select Assay Method ORAC (Oxygen Radical Absorbance Capacity)TEAC (Trolox Equivalent Antioxidant Capacity)FRAP (Ferric Reducing Antioxidant Power) Sample Antioxidant Value Unit µmol TE/g (dry weight)µmol TE/100g (fresh weight)mmol Fe²⁺/g Calculate Antioxidant Capacity Results About the Antioxidant Capacity Calculator The Antioxidant Capacity Calculator is a scientifically validated tool that measures the total antioxidant power

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