All Biochemistry Calculators Related Post

    Steroid Binding Calculator Predict Relative Binding Affinity (RBA %) of any steroid or analog to nuclear hormone receptors using validated structure-activity relationship (SAR) models from peer-reviewed literature. Select Base Steroid Testosterone (reference)Dihydrotestosterone (DHT)Nandrolone (19-nortestosterone)BoldenoneTrenboloneEstradiol (E2)ProgesteroneCortisolCustom Analog (manual input) Target Receptor Androgen Receptor (AR)Estrogen Receptor (ERα/β)Progesterone Receptor (PR)Glucocorticoid Receptor (GR)Mineralocorticoid Receptor (MR) 17α/β Modification […]

    Nuclear Receptor Calculator Predict ligand-binding affinity, receptor activation potency (EC50 estimate), selectivity profile, and transcriptional outcome for all 48 human nuclear receptors using validated scientific models. Select Nuclear Receptor Estrogen Receptor α (ERα)Estrogen Receptor β (ERβ)Androgen Receptor (AR)Glucocorticoid Receptor (GR)Mineralocorticoid Receptor (MR)Progesterone Receptor (PR)PPARγ (Peroxisome Proliferator-Activated Receptor γ)PPARαPPARδLiver X Receptor α (LXRα)Liver X

Transcription Factor Calculator The Transcription Factor Calculator is an advanced online tool designed to predict the fractional occupancy and transcriptional activation level of a DNA-binding site by a transcription factor based on real biophysical principles. Using the well-established Hill-Langmuir binding equation widely accepted in molecular biology and systems biology research, this calculator helps students, researchers,

    Histone Acetylation Calculator Accurately estimate the degree of histone acetylation based on experimental ChIP-seq, ATAC-seq, or biochemical assay data using peer-reviewed scientific formulas. Total Histone H3/H4 Amount (pmol or ng) Acetylated Histone Amount (pmol or ng) Assay Type Western Blot / Mass SpecChIP-seq / ChIP-qPCRAcid Extraction + ELISA Specific Acetylation Site (Optional) Global

    Histone Methylation Calculator Predict histone methylation patterns (H3K4me3, H3K27me3, H3K9me3, H3K36me3) from gene expression, genomic context, and chromatin features using validated ENCODE & Roadmap Epigenomics models. Gene Expression Status Highly Expressed (Housekeeping)Moderately ExpressedLow / Tissue-SpecificTranscriptionally Silent Genomic Location Near Transcription Start Site (±2kb)Proximal PromoterEnhancer RegionGene BodyIntergenic / Repetitive CpG Content at Promoter High

    Methylation Pattern Calculator Methylation Pattern Calculator is a free, scientifically accurate online tool designed to analyze DNA methylation patterns from bisulfite-treated sequencing data. DNA methylation, especially at CpG sites, is one of the most important epigenetic modifications influencing gene expression, genomic imprinting, X-chromosome inactivation, and disease development including cancer. This Methylation Pattern Calculator

    Epigenetic Regulation Calculator The Epigenetic Regulation Calculator is a scientifically accurate, free online tool designed to estimate the combined impact of major epigenetic mechanisms — DNA methylation, histone modifications, and non-coding RNA regulation — on gene expression silencing or activation probability. Built strictly on peer-reviewed models published in Nature Reviews Genetics, Cell, and

    Gene Silencing Calculator Accurately predict the silencing efficiency of your siRNA, shRNA, or miRNA using the most trusted peer-reviewed algorithms (Reynolds 2004, Ui-Tei 2004, Hsieh 2004, Amarzguioui 2004). siRNA Sense Strand Sequence (19–21 nt) e.g., GAUGUCAAGCUGUCAAGAU Target Organism Human / MammalianMouse / RatPlant (Arabidopsis, Rice, etc.)Insect (Drosophila)Other Position on Target mRNA Coding Sequence

    siRNA Efficiency Calculator Accurately predict siRNA knockdown efficiency using the most trusted peer-reviewed algorithms (Reynolds 2004, Ui-Tei 2004, Amarzguioui 2004, Huesken 2005). Sense Strand Sequence (19–21 nt) e.g., GAUGCUUACGUUAGCUUCU Antisense Strand Sequence (optional) Leave blank for auto-generation Target Position in mRNA Coding Sequence (CDS)3′ UTR5′ UTR (not recommended) GC Content (%) Leave blank

    Guide RNA Design Calculator Guide RNA Design Calculator is a free, scientifically accurate online tool that helps researchers and students design highly efficient and specific CRISPR-Cas9, Cas12a, or base-editor guide RNAs (gRNA) sequences. This calculator uses peer-reviewed scoring algorithms (Doench 2016, Hsu 2013, Moreno-Mateos 2015, CFD specificity score) to predict on-target efficacy, off-target