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Protein Migration Calculator

Protein Migration Calculator

Protein Migration Calculator Calculate Protein Migration Distance Enter the protein molecular weight and gel conditions to estimate migration distance. Protein Molecular Weight (kDa): Gel Type: Polyacrylamide (SDS-PAGE)Agarose Gel Percentage (%): Run Time (minutes): Voltage (V): Calculate Result About the Protein Migration Calculator The Protein Migration Calculator is a powerful tool designed to help researchers, students, […]

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Western Blot Calculator

Western Blot Calculator

Western Blot Calculator Calculate Protein Expression Enter the densitometry values to quantify protein expression relative to a housekeeping protein. Target Protein Density (arbitrary units): Housekeeping Protein Density (arbitrary units): Control Target Protein Density (arbitrary units): Control Housekeeping Protein Density (arbitrary units): Calculate Relative Expression: Fold Change: About the Western Blot Calculator The Western Blot Calculator

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SDS-PAGE Calculator

SDS-PAGE Calculator

SDS-PAGE Calculator Calculate Protein Molecular Weight Enter the relative mobility (Rf) values and known molecular weights of standard proteins, then provide the Rf value of the unknown protein to estimate its molecular weight. Standard Proteins (Molecular Weight in kDa, Rf value; one per line): Unknown Protein Rf Value: Calculate About the SDS-PAGE Calculator The SDS-PAGE

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Gel Electrophoresis Calculator

Gel Electrophoresis Calculator

Gel Electrophoresis Calculator Calculate DNA Migration Distance DNA Fragment Size (bp): Agarose Gel Percentage (%): 0.7%1.0%1.5%2.0%3.0% Voltage Applied (V): Run Time (minutes): Calculate Migration Distance About the Gel Electrophoresis Calculator The Gel Electrophoresis Calculator is a specialized tool designed to assist researchers, students, and professionals in molecular biology and biochemistry. This calculator estimates the migration

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Affinity Chromatography Calculator

Affinity Chromatography Calculator

Affinity Chromatography Calculator Calculate Chromatography Parameters Ligand Density (µmol/mL): Column Volume (mL): Protein Concentration (mg/mL): Sample Volume (mL): Elution Volume (mL): Calculate Binding Capacity: mg Purification Yield: % Elution Concentration: mg/mL About the Affinity Chromatography Calculator The Affinity Chromatography Calculator is a specialized tool designed to assist researchers, scientists, and students in calculating critical parameters

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Size Exclusion Calculator

Size Exclusion Calculator

Size Exclusion Calculator Calculate Elution Volume or Molecular Weight Void Volume (V₀, mL)?The volume of the mobile phase outside the gel pores. Total Column Volume (Vₜ, mL)?The total volume of the column, including gel pores. Elution Volume (Vₑ, mL, optional)?The volume at which the molecule elutes (leave blank to calculate). Molecular Weight (kDa, optional)?The molecular

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Gradient Elution Calculator

Gradient Elution Calculator

Gradient Elution Calculator Calculate Gradient Parameters Column Holdup Time (tM, min): Gradient Time (tG, min): Flow Rate (F, mL/min): Gradient Range (Δφ, %): S-Value (average change in log k): Initial Retention Factor (ko): Dwell Time (tD, min): Calculate Results About the Gradient Elution Calculator The Gradient Elution Calculator is a powerful tool designed to assist

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HPLC Retention Time Calculator

HPLC Retention Time Calculator

    HPLC Retention Time Calculator Calculate Retention Time Retention Factor (k): Column Dead Time (t0, minutes): Organic Modifier: MethanolAcetonitrile Calculate About the HPLC Retention Time Calculator The HPLC Retention Time Calculator is a specialized tool designed to assist researchers, scientists, and analytical chemists in predicting the retention time of compounds in High-Performance Liquid Chromatography

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Column Volume Calculator

Column Volume Calculator

Column Volume Calculator Column Diameter (mm): Column Length (mm): Calculate Volume About the Column Volume Calculator The Column Volume Calculator is a precise tool designed to compute the volume of a cylindrical column, commonly used in scientific applications such as chromatography. This calculator employs the scientifically verified formula for the volume of a cylinder, V

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Protein Yield Calculator

Protein Yield Calculator

Protein Yield Calculator Sample Weight (g): Protein Concentration (mg/g): Extraction Efficiency (%): Calculate Protein Yield About the Protein Yield Calculator The Protein Yield Calculator is a scientifically designed tool that enables researchers, biochemists, and agricultural professionals to estimate the amount of protein extracted from a given sample. This tool is grounded in established biochemical principles

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