All Biochemistry Calculators Related Post

    Epitope Prediction Calculator Predict B-cell & T-cell Epitopes Paste your protein sequence in FASTA or plain format (single-letter amino acid codes only). Protein Sequence MHC Allele (for T-cell epitope prediction) HLA-A*02:01 (most common)HLA-A*01:01HLA-A*03:01HLA-A*24:02HLA-B*07:02HLA-B*08:01 Peptide Length (for MHC-I) 9-mer (most common)8-mer10-mer11-mer Calculate Epitopes About the Epitope Prediction Calculator The Epitope Prediction Calculator is a […]

    MHC Binding Calculator Predict MHC Class I Peptide Binding Affinity Peptide Sequence (8–14 mers, single-letter code) HLA Allele HLA-A*02:01 (most common)HLA-A*01:01HLA-A*03:01HLA-A*11:01HLA-A*24:02HLA-B*07:02HLA-B*08:01HLA-B*35:01HLA-B*44:02HLA-C*07:02 Prediction Method (2025 updated) NetMHCpan-4.1 EL (most accurate)NetMHCpan-4.0IEDB Consensus Binding Threshold Strong binder ≤0.5% RankWeak binder ≤2.0% Rank Calculate MHC Binding Clear All About the MHC Binding Calculator The MHC Binding Calculator

Peptide Antigenicity Calculator Calculate Peptide Antigenicity Score Enter Peptide Sequence (single-letter code) Antigenicity Prediction Method Parker Hydrophilicity (1986) – Most widely usedKolaskar & Tongaonkar (1990) – B-cell epitopeEmini Surface Accessibility (1985)Chou & Fasman Beta-Turn (1978) Window Size 7 residues (recommended & standard)5 residues6 residues8 residues9 residues Calculate Antigenicity Clear About the Peptide Antigenicity Calculator The

    Protein Fragment Calculator The Protein Fragment Calculator is an advanced, scientifically accurate online tool designed for researchers, biochemists, and molecular biologists to quickly calculate critical parameters of protein fragments used in Protein-fragment Complementation Assays (PCA), split-protein systems, and protein engineering projects. This Protein Fragment Calculator enables precise determination of molecular weight, isoelectric point

    Protease Specificity Calculator About the Protease Specificity Calculator The Protease Specificity Calculator is a free, scientifically accurate online tool that predicts how likely a given peptide sequence is to be cleaved by major human proteases using position-specific scoring matrices (PSSMs) derived from experimentally validated cleavage data published in peer-reviewed literature (MEROPS database and

Peptide Cleavage Calculator Enter Protein Sequence & Select Protease Protein Sequence (single-letter code) Numbers, spaces and lowercase will be automatically cleaned Select Protease / Enzyme Trypsin (cuts after K, R – not before P)Chymotrypsin (cuts after F, Y, W – not before P)Pepsin pH>2 (cuts after F, L – not before P)Thermolysin (cuts before L,

Cleavage Site Calculator Predict Cleavage Sites in Your Protein Sequence Protein Sequence (single-letter code) Select Protease / Cleavage Rule Trypsin (K,R | not before P)Chymotrypsin (F,Y,W | not before P)Pepsin pH>2 (F,L | hydrophobic)Thermolysin (before A,L,V,I,M,F)Cyanogen Bromide (M | methionine)Asp-N (before D)Glu-C (E | in phosphate buffer)Lys-C (K | not before P)Arg-C (R | not

    Skin Tag Removal Calculator Quickly estimate the safest and most cost-effective skin tag removal method based on size, location, quantity, and your skin type. This Skin Tag Removal Calculator uses peer-reviewed medical guidelines (2023–2025) to give you personalized recommendations. Number of Skin Tags Average Size (mm) ≤ 2 mm (tiny)2–5 mm (small)5–10 mm

GFP Fusion Calculator Design Your GFP Fusion Protein Protein of Interest Sequence (single-letter code) Numbers, spaces and invalid characters will be removed automatically Fusion Type N-terminal GFP Fusion (GFP–Protein)C-terminal GFP Fusion (Protein–GFP)Internal Fusion (specify position below) Insertion Position (residue number after which GFP is inserted) Linker Sequence (optional) No linkerFlexible (GGGGS)Flexible 2× (GGGGS)₂Flexible 3× (GGGGS)₃Rigid

    Fluorescent Tag Calculator Free Online Fluorescent Tag Calculator Calculate optimal labeling ratios, degree of labeling (DOL), and required amounts for protein/antibody fluorescent tagging Protein / Antibody Molecular Weight (kDa) Fluorescent Dye Molecular Weight (Da) Protein Concentration (mg/mL) Dye Concentration (mg/mL or mM) mg/mLmM Target Degree of Labeling (DOL) Reaction Volume (µL) Calculate Fluorescent