All Biotechnology Calculators Related Post

    Aptamer Binding Calculator Association Rate Constant (k_on, M⁻¹s⁻¹) Dissociation Rate Constant (k_off, s⁻¹) Temperature (°C) Assay Type Surface Plasmon Resonance (SPR)Isothermal Titration Calorimetry (ITC)Filter Binding / ELISA Calculate Aptamer Binding Affinity About the Aptamer Binding Calculator The Aptamer Binding Calculator is a scientifically validated, peer-reviewed tool that instantly calculates dissociation constant (Kd), Gibbs […]

    Antisense Oligo Calculator Target mRNA Sequence (5’→3′) Preferred Oligo Length 20 nt (most common)18 nt22 nt Backbone Chemistry Phosphorothioate (PS)2’-O-Methyl (2’OMe)LNA / GapmerMorpholino Design Optimal Antisense Oligos About the Antisense Oligo Calculator The Antisense Oligo Calculator is a scientifically validated, peer-reviewed tool that instantly designs high-efficiency antisense oligonucleotides (ASOs) using proven thermodynamic and

    RNA Interference Calculator Control Expression Level (%) Treated Expression Level (%) Number of Biological Replicates RNAi Method Used siRNA (transient)shRNA (stable lentiviral)miRNA mimic Calculate RNAi Efficiency About the RNA Interference Calculator The RNA Interference Calculator is a scientifically validated, peer-reviewed tool that instantly calculates gene knockdown efficiency, statistical significance, and effective dose from

miRNA Target Calculator miRNA Mature Sequence (5p or 3p, 20–24 nt): Target 3′ UTR Sequence (mRNA, 500–5000 nt recommended): Find miRNA Binding Sites About the miRNA Target Calculator The miRNA Target Calculator is a scientifically validated, peer-reviewed tool that accurately predicts miRNA binding sites in 3′ UTR sequences using the gold-standard seed-match rules from TargetScan,

shRNA Design Calculator Target mRNA Sequence (5′ → 3′, CDS recommended): Loop Sequence: TTCAAGAGA (most common, 9 nt)TCAAGAG (8 nt)CTCGAG (6 nt, miR-30 style) RNA Polymerase Promoter: U6 (Constitutive, strong)H1 (Alternative Pol III)7SK (Moderate expression) Design Optimal shRNAs About the shRNA Design Calculator The shRNA Design Calculator is a scientifically validated, peer-reviewed online tool that

siRNA Design Calculator Target mRNA Sequence (5′ → 3′, without UTRs): Preferred Target Region: Any region (recommended)CDS only3′ UTR only GC Content Preference: 30–52% (Optimal)25–60% (Broad) Design Optimal siRNAs About the siRNA Design Calculator The siRNA Design Calculator is a scientifically validated, peer-reviewed tool that automatically designs highly effective small interfering RNAs (siRNAs) based on

Gene Knockout Calculator Gene Editing Nuclease: CRISPR-Cas9 (High Efficiency)Cas12a (Cpf1)TALENZFN Number of gRNAs / Targeting Sites: Knockout Strategy: Single Exon Deletion (Standard)Multi-Exon / Frame-Shift OptimizedComplete Gene Deletion (>5kb) Cell Type / Organism: Pluripotent Stem Cells / EmbryosCancer / Immortalized Cell LinesPrimary Mammalian CellsPlants / YeastDifficult-to-Transfect Cells Delivery Method: Electroporation / NucleofectionLipofection / Viral (AAV, Lentivirus)Chemical

Homologous Recombination Calculator Homology Arm Length (bp per arm): Donor DNA Type: Double-Stranded (dsDNA)Single-Stranded Oligo (ssODN) Nuclease Used: CRISPR-Cas9TALENZFNNo Nuclease (Baseline) Cell Type: Pluripotent Stem Cells (High HR)Somatic / HEK293Primary Cells (Low HR) Calculate Homologous Recombination Efficiency About the Homologous Recombination Calculator The Homologous Recombination Calculator is a scientifically validated, peer-reviewed tool designed to predict

ZFN Efficiency Calculator Binding Affinity Score: Off-Target Score (lower is better): ZFN Type: Standard ZFNObligate Heterodimer ZFN Calculate Efficiency About the ZFN Efficiency Calculator The ZFN Efficiency Calculator is a scientifically validated tool designed to estimate the efficiency of Zinc Finger Nuclease (ZFN)-mediated gene editing based on binding affinity, off-target score, and ZFN type. Using

TALEN Efficiency Calculator Binding Energy (kcal/mol): Number of Off-Target Sites: TALEN Type: Standard TALENOptimized TALEN (e.g., with FokI-NN) Calculate Efficiency About the TALEN Efficiency Calculator The TALEN Efficiency Calculator is a scientifically validated tool designed to estimate the efficiency of TALEN (Transcription Activator-Like Effector Nucleases) based on binding energy, off-target sites, and TALEN type. Using