All Biotechnology Calculators Related Post

siRNA Design Calculator Target mRNA Sequence (5′ → 3′, without UTRs): Preferred Target Region: Any region (recommended)CDS only3′ UTR only GC Content Preference: 30–52% (Optimal)25–60% (Broad) Design Optimal siRNAs About the siRNA Design Calculator The siRNA Design Calculator is a scientifically validated, peer-reviewed tool that automatically designs highly effective small interfering RNAs (siRNAs) based on […]

Gene Knockout Calculator Gene Editing Nuclease: CRISPR-Cas9 (High Efficiency)Cas12a (Cpf1)TALENZFN Number of gRNAs / Targeting Sites: Knockout Strategy: Single Exon Deletion (Standard)Multi-Exon / Frame-Shift OptimizedComplete Gene Deletion (>5kb) Cell Type / Organism: Pluripotent Stem Cells / EmbryosCancer / Immortalized Cell LinesPrimary Mammalian CellsPlants / YeastDifficult-to-Transfect Cells Delivery Method: Electroporation / NucleofectionLipofection / Viral (AAV, Lentivirus)Chemical

Homologous Recombination Calculator Homology Arm Length (bp per arm): Donor DNA Type: Double-Stranded (dsDNA)Single-Stranded Oligo (ssODN) Nuclease Used: CRISPR-Cas9TALENZFNNo Nuclease (Baseline) Cell Type: Pluripotent Stem Cells (High HR)Somatic / HEK293Primary Cells (Low HR) Calculate Homologous Recombination Efficiency About the Homologous Recombination Calculator The Homologous Recombination Calculator is a scientifically validated, peer-reviewed tool designed to predict

ZFN Efficiency Calculator Binding Affinity Score: Off-Target Score (lower is better): ZFN Type: Standard ZFNObligate Heterodimer ZFN Calculate Efficiency About the ZFN Efficiency Calculator The ZFN Efficiency Calculator is a scientifically validated tool designed to estimate the efficiency of Zinc Finger Nuclease (ZFN)-mediated gene editing based on binding affinity, off-target score, and ZFN type. Using

TALEN Efficiency Calculator Binding Energy (kcal/mol): Number of Off-Target Sites: TALEN Type: Standard TALENOptimized TALEN (e.g., with FokI-NN) Calculate Efficiency About the TALEN Efficiency Calculator The TALEN Efficiency Calculator is a scientifically validated tool designed to estimate the efficiency of TALEN (Transcription Activator-Like Effector Nucleases) based on binding energy, off-target sites, and TALEN type. Using

Gene Editing Efficiency Calculator Total Cells Edited (in millions): Successfully Edited Cells (in millions): Gene Editing Method: CRISPR-Cas9 (Standard Efficiency)TALEN (Moderate Efficiency)ZFN (Lower Efficiency) Off-Target Mutation Rate (%): Calculate Efficiency About the Gene Editing Efficiency Calculator The Gene Editing Efficiency Calculator is a scientifically validated tool designed to estimate the efficiency of gene editing experiments

Transfection Yield Calculator Number of Cells (in millions): DNA Amount (µg): Transfection Efficiency (%): Transfection Method: Lipofection (Standard Efficiency)Electroporation (High Cell Stress)Calcium Phosphate (Moderate Efficiency) Calculate Transfection Yield About the Transfection Yield Calculator The Transfection Yield Calculator is a scientifically validated tool designed to estimate the number of successfully transfected cells in a molecular biology

Molecular Cloning Calculator Vector Size (base pairs): Insert Size (base pairs): Vector Concentration (ng/µL): Desired Vector:Insert Molar Ratio: 1:13:15:1 Calculate Cloning Parameters About the Molecular Cloning Calculator The Molecular Cloning Calculator is a scientifically validated tool designed to optimize ligation reactions in molecular cloning by calculating the amount of DNA insert needed based on vector

    Protein Fragment Size Calculator Migration Distance (mm): Standard 1 Distance (mm): Standard 1 MW (kDa): Standard 2 Distance (mm): Standard 2 MW (kDa): Calculate Fragment Size About the Protein Fragment Size Calculator The Protein Fragment Size Calculator is a reliable scientific tool for estimating protein fragment molecular weights (MW) from SDS-PAGE gel migration

    RNA Fragment Size Calculator Migration Distance (mm): Standard 1 Distance (mm): Standard 1 Size (nt): Standard 2 Distance (mm): Standard 2 Size (nt): Calculate Fragment Size About the RNA Fragment Size Calculator The RNA Fragment Size Calculator is a scientifically accurate tool designed to estimate RNA fragment sizes from gel electrophoresis migration distances.