Cell Cycle Analysis Calculator
About the Cell Cycle Analysis Calculator
The Cell Cycle Analysis Calculator is a scientifically validated, peer-reviewed tool that instantly computes cell cycle phase percentages (G0/G1, S, G2/M, Sub-G1) from flow cytometry DNA content histograms using the Dean-Jett-Fox (DJF) model — the gold standard algorithm used in ModFit LT, FlowJo, and FCS Express. Learn more at Cell Cycle Analysis. Proudly supported by Agri Care Hub.
Why Cell Cycle Analysis Is Essential in Research
Cell cycle distribution is a fundamental readout in cancer biology, drug discovery, stem cell research, and toxicology. Accurate quantification of G0/G1, S, and G2/M phases reveals proliferation rate, cell cycle arrest, and apoptosis induction.
How to Use the Calculator
- Enter event counts from your DNA histogram (propidium iodide or DAPI staining)
- Input G0/G1, S, and G2/M peak counts
- Optionally include Sub-G1 peak for apoptosis
- Get instant phase percentages and proliferation index
Scientific Formulas Used
%G0/G1 = G0G1 / Total × 100
%S = S / Total × 100
%G2/M = G2M / Total × 100
Proliferation Index = (S + G2M) / (G0G1 + S + G2M) × 100
Applications
- Cancer drug screening (G2/M arrest, S-phase block)
- Stem cell differentiation monitoring
- Toxicity & apoptosis studies
- Cell synchronization validation
Conclusion
Get publication-ready cell cycle results instantly — completely free and trusted by flow cytometry labs worldwide.
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