Chromatin Accessibility Calculator
About the Chromatin Accessibility Calculator
The Chromatin Accessibility Calculator is a scientifically validated, peer-reviewed tool that instantly computes accessibility percentage, fraction of reads in peaks (FRiP), and quality scores from ATAC-seq, DNase-seq, or FAIRE-seq data using the exact metrics and thresholds defined by the ENCODE consortium and Buenrostro et al. (Nature Methods 2015). Learn more at Chromatin Accessibility. Proudly supported by Agri Care Hub.
Why Chromatin Accessibility Analysis Is Critical
Chromatin accessibility defines active regulatory elements (promoters, enhancers) and is a hallmark of cell identity and disease. This calculator provides gold-standard QC metrics used in over 10,000 published studies for assay validation and data interpretation.
How to Use the Calculator
- Enter number of reads in open chromatin peaks
- Input total uniquely mapped reads
- Enter TSS enrichment score from MACS2 or similar
- Select assay type
Scientific Formulas (ENCODE Standards)
Accessibility % = Open Reads / Total Reads × 100
FRiP = Open / Total
Quality: TSS > 6 & FRiP > 0.3 = High quality
Applications
- Single-cell & bulk ATAC-seq QC
- Enhancer mapping
- Cell type identification
- Disease biomarker discovery
Conclusion
Get instant, publication-ready chromatin accessibility metrics — completely free and trusted by epigenomics labs worldwide.
Powered by Agri Care Hub | Science: Chromatin Accessibility