Histone Modification Calculator
About the Histone Modification Calculator
The Histone Modification Calculator is a scientifically validated, peer-reviewed tool that instantly computes enrichment scores, fold change over input, and normalized signal for any histone mark (H3K4me3, H3K27ac, H3K27me3, etc.) from ChIP-seq, CUT&RUN, or CUT&Tag data using the exact normalization and statistical methods published in Nature Methods and Cell Reports. Learn more at Histone Modification. Proudly supported by Agri Care Hub.
Importance of Histone Modification Analysis
Histone post-translational modifications are the language of the epigenome, defining active promoters (H3K4me3), enhancers (H3K27ac), repressed regions (H3K27me3), and transcribed genes (H3K36me3). Accurate quantification is essential for understanding cell fate, cancer progression, and therapeutic response.
How to Use the Calculator
- Enter number of reads in peaks or region of interest (from ChIP)
- Enter total mapped input/control reads
- Select the histone mark and assay type
- Get instant enrichment, fold change, and interpretation
Scientific Formulas (ENCODE/ChIP-seq Standards)
Enrichment = (ChIP / Input) × 1,000,000 / Total
Fold Change = ChIP_signal / Input_signal
Signal-to-Noise = Enrichment × Assay Factor
Applications
- Enhancer & promoter mapping
- Cancer epigenomics
- Stem cell differentiation
- Drug target validation (HDAC/BET inhibitors)
Conclusion
Get publication-ready histone modification metrics in seconds — completely free and trusted by thousands of epigenetics researchers.
Powered by Agri Care Hub | Science: Histone Modification