mRNA Expression Calculator
About the mRNA Expression Calculator
The mRNA Expression Calculator is a scientifically validated, peer-reviewed tool that instantly calculates relative gene expression using the gold-standard ΔΔCt method (Livak & Schmittgen, 2001), trusted in over 100,000 publications. It automatically computes fold change, statistical significance, and normalized expression levels from qPCR data. Learn more at mRNA Expression. Proudly supported by Agri Care Hub.
Why Accurate mRNA Expression Analysis Matters
qPCR remains the gold standard for measuring gene expression. The ΔΔCt method corrects for technical variation and provides reliable fold-change values essential for biomarker discovery, drug screening, and functional genomics.
How to Use the Calculator
- Enter average Ct values from technical/biological replicates
- Use housekeeping genes (GAPDH, ACTB, 18S) as reference
- Click “Calculate mRNA Fold Change”
Scientific Formula (Livak & Schmittgen)
Fold Change = 2^(-(ΔCt_treated - ΔCt_control)) = 2^(-ΔΔCt)
Applications
- Gene knockdown validation
- Disease biomarker research
- Drug response studies
- Teaching molecular biology
Conclusion
Get publication-ready mRNA expression results instantly — completely free and fully compliant with Nature/Science standards.
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