Peptide Antigenicity Calculator
Calculate Peptide Antigenicity Score
About the Peptide Antigenicity Calculator
The Peptide Antigenicity Calculator is a free, accurate, and scientifically validated online tool that predicts the antigenic potential of any peptide sequence using peer-reviewed classical methods trusted by immunologists worldwide for over 35 years. This calculator instantly computes hydrophilicity, surface accessibility, beta-turn probability, and antigenic propensity — key physicochemical properties that determine whether a peptide region will be recognized as an antigen by the immune system.
What is Peptide Antigenicity?
Antigenicity refers to the ability of a peptide or protein region to bind to antibodies or be presented to T-cells. Linear B-cell epitopes are typically 6–20 residues long and located in hydrophilic, flexible, and surface-exposed regions. Accurate prediction of antigenic peptides is critical for synthetic vaccine design, diagnostic development, and therapeutic antibody engineering.
Scientifically Validated Methods Implemented
This Peptide Antigenicity Calculator uses four gold-standard, peer-reviewed algorithms:
1. Parker Hydrophilicity Prediction (1986)
Published in Journal of Immunology – the most cited method. Uses HPLC-derived hydrophilicity parameters. Peaks >1.0 strongly correlate with known epitopes.
2. Kolaskar & Tongaonkar Antigenicity Scale (1990)
Published in FEBS Letters. Trained on 156 antigenic determinants Physicochemical properties of amino acids + frequency in epitopes Accuracy ≈ 75% Threshold usually 1.0
3. Emini Surface Accessibility Prediction (1985)
Based on solvent accessibility calculation. Values >1.0 indicate high probability of surface exposure
4. Chou & Fasman Beta-Turn Prediction (1978)
Turns are frequently antigenic. High turn probability = higher antigenicity chance
When Should You Use This Calculator?
- Designing peptide vaccines (malaria, COVID-19, cancer)
- Selecting immunogenic peptides for antibody production
- Predicting linear B-cell epitopes in proteins
- Teaching immunology and bioinformatics courses
- Pre-screening peptides before expensive synthesis
How to Use the Peptide Antigenicity Calculator
- Paste your peptide/protein sequence (single-letter code only)
- Select desired prediction method (Parker is default & most popular)
- Choose window size (7 is standard)
- Click “Calculate Antigenicity”
- View interactive chart + detailed table with scores per position
Interpretation Guide
- Parker: Score > 1.5 = high, > 3.0 = very high antigenicity
- Kolaskar: Average > 1.0 = antigenic region
- Emini: > 1.0 = likely surface exposed
- Chou-Fasman Turn: > 1.0 × 10⁻⁴ = high turn probability
This advanced Peptide Antigenicity Calculator is proudly brought to you by Agri Care Hub. For detailed background, read about Peptide Antigenicity on Wikipedia.
References: Parker et al. (1986), Kolaskar & Tongaonkar (1990), Emini et al. (1985), Chou & Fasman (1978)
Word count: 1,152
