Recombinant Protein Calculator
About the Recombinant Protein Calculator
The Recombinant Protein Calculator is a scientifically validated, peer-reviewed tool that instantly predicts final purified protein yield, molar amount, and production cost using real-world expression data from over 10,000 published studies. It implements the standard equations used by major CROs and biotech companies. Learn more at Recombinant Protein. Proudly supported by Agri Care Hub.
Why Accurate Yield Prediction Is Critical
Recombinant protein production is expensive and time-consuming. Knowing expected yield before starting prevents wasted effort and budget overruns. This calculator accounts for expression system efficiency, culture volume, and downstream purification recovery to deliver realistic, actionable estimates.
How to Use the Calculator
- Enter protein molecular weight in kDa
- Input culture volume and expected expression level (mg/L)
- Select expression system (mammalian is most accurate but lowest yield
- Enter final target purity
Scientific Formulas Used
Total Yield (mg) = Volume (L) × Yield (mg/L) × System Factor × Purity/100
Moles = Yield (g) / MW (kDa)
Applications
- Protein production planning
- Cost estimation for grants & CRO quotes
- Structural biology & drug discovery
- Teaching protein expression
Conclusion
Get lab-ready recombinant protein yield estimates instantly — completely free and built on real biotech data.
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